Piracetam bioequivalence study

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Piracetam bioequivalence study

Posted Apr 04, 2016 by Admin

Mobile phase was used as extraction solvent as well. 2.4. Analytical Procedure. Standard Preparation 10 mg weight each of piracetam and levetiracetam was taken in a 100 ml volumetric flask separately. 50 mL of the diluent was added, sonicated for few minutes and stirred for 30 minutes on. PMID : PubMed - indexed for MEDLINE. MeSH Terms, Substances MeSH Terms Acetonitriles/chemistry Chromatography, High Pressure Liquid/methods. Humans Methanol/chemistry Piracetam/analysis Piracetam/blood Sensitivity and Specificity Substances. Acetonitriles Methanol acetonitrile Piracetam LinkOut - more resources).

Prior to use mobile phase was filtered through a m membrane filter (Millipore, Bedford, MA, USA). Flow rate was 1.0 mL/min Injection, injected volume was L, and the detecting wavelength was 205 nm.

After which scheduled breakfast, lunch, and dinner were provided to all.

RP-HPLC ultraviolet detection simultaneous quantification of piracetam and levetiracetam has been developed and validated. The chromatography was obtained on a Nucleosil C18 column of, 10 m, dimension. The mobile phase was a (70 : 30 v/v) mixture of 0.1 g/L of triethylamine and acetonitrile.

1Faculty of Pharmacy, Federal Urdu University Arts, Science and Technology, Karachi 75300, Pakistan 2Faculty of Pharmacy, Ziauddin University, Karachi 75600, Pakistan 3Department of Chemistry, University of Karachi, Karachi 75270, Pakistan 4Department of Environmental Sciences, Federal Urdu University Arts, Science and Technology, Karachi 75300, Pakistan 5Faculty.

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Smooth flow of mobile phase at 1 mL/min was set and 205 nm wavelength was selected. Results were evaluated through statistical parameters which qualify the method reproducibility and selectivity for the quantification of piracetam, levetiracetam, and their impurities hence proving stability-indicating properties.

It is a nootropic, psychopharmacological drug. It is believed that it has a role in augmenting cognition and memory, impeding brain disaster, and improving blood and oxygen flow to the brain.

Stability studies suggest proper formulations, design manufacturing processes, and selecting proper storage condition and packaging for the drug product. Furthermore, it helps in establishing shelf life of product 3 5. All these make it highly important to have a stability indicating method in hand.

2 mL of plasma was mixed with 2 mL of acetonitrile and 2 mL of drug solution was shaken, vortexed for 5 min, and centrifuged at 3000 rpm for 10 min. supernatant clear liquid was mixed from both the drugs and was appropriately diluted to get working range concentrations of piracetam.

2. Experimental 2.1. Materials and Reagents. Piracetam and levetiracetam were kind gifts from National Pharmaceuticals, sulphuric acid was purchased from Merck (Germany and methanol (HPLC grade) was from Fisher Scientific. The tablets containing piracetam and levetiracetam were purchased from home market.

The sample weight equivalent to 100 mg of piracetam and levetiracetam was accurately taken in 100 mL volumetric flask, followed by the addition of 50 mL of diluent. The sample after sonication for 10 minutes was placed for stirring for 20 minutes.

Copyright 2014 Farhan Ahmed Siddiqui et al. This is an open access article distributed under the. Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Marinescu Str. No.38, Tg-Mures RO-540139, Romania. Abstract The new method for HPLC determination of piracetam in human plasma was developed and validated by a new approach. The simple determination by UV detection was performed on supernatant, obtained from plasma, after proteins precipitation with perchloric acid.